ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set

General description

Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.

Specificity

Recognizes LEF1 (lymphoid enhancer-binding factor 1).

Immunogen

The antibody is made against amino acid residues 1-85 of human LEF1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Transcription Factors

Use ChIPAb+ LEF1 Mouse Monoclonal Antibody validated in WB, ChIP for the detection of LEF1.

Western Blot Analysis:
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 mg/mL). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

Packaging

25 assays per kit. ~4μg per chromatin immunoprecipitation.

Quality

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 3x10⁶ HT29 cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Mouse Normal IgG or mouse Anti-LEF1 and the Magna ChIP^® G kit (Cat.# 17-611).
Successful enrichment of LEF1 associated DNA fragments was verified by qPCR using ChIP Primers c-myc flanking the human c-myc promoter that contains LEF1 binding sites (Please see figures).
Please refer to the EZ-Magna ChIP G (Cat.# 17-409) or EZ-ChIP (Cat.# 17-371) kit protocols for experimental details.

Target description

55-57 kDa

Physical form

Anti-LEF1 (mouse IgG1). 1 vial containing 100 mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100 mL of phosphate buffered saline with sodium azide. Store at-20°C. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4.

Normal Mouse IgG. One vial containing 125 μg of normal mouse IgG in 125 μL volume. Store at -20°C.

ChIP primers c-myc. One vial containing 75 mL of 5 μM of each control primer specific for human c-myc promoter. Store at -20°C.
FOR: CCC AAA AAA AGG CAC GGA A
REV: TAT TGG AAA TGC GGT CAT GC

Format: Purified

Storage and Stability

1 year at -20°C from date of shipment

Analysis Note

Control
Includes negative control mouse IgG antibody and control primers specific for human c-myc promoter.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.