Cholesterol Esterase from porcine pancreas (C15-1291-434)

General description

Cholesterol Esterase is a serine hydrolase released from the exocrine pancreas. It belongs to the α/β hydrolase family of enzymes.

Application

Cholesterol Esterase from porcine pancreas has been used:

• in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase
• to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model
• as a standard in enzyme activity assay

Biochem/physiol Actions

Cholesterol esterase exhibits broad substrate specificity and regulates the bile salt-mediated hydrolysis of dietary cholesteryl esters. It participates in the hydrolysis of triglycerides and phospholipids which are essential for normal cholesterol absorption. Cholesterol esterase plays a key role in transporting free cholesterol to enterocytes. Loss of enzyme function reduces the intestinal absorption of dietary cholesteryl esters.

For the hydrolysis of cholesterol esters in the determination of total cholesterol in serum or plasma; Application in (selective) hydrolysis/condensation of carboxylic ester bonds

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol cholesterol per minute at pH 7.0 and 37°C (cholesterol acetate as substrate)

Other Notes

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