Cholesterol Quantification Assay kit

General description

Cholesterol is a lipid that constitutes 20-25% of the structural components of cell membranes. Cholesterol presence assure membrane structural fluidity. Cholesterol also plays a role as an intermediate in several hormones and bile acid biosynthesis and vitamin D. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane. The major sites of cholesterol synthesis are the small intestine and liver. Cholesterol circulates in the blood as both the free acid and as cholesterol esters. Increased cholesterol levels are associated with the development of atherosclerosis and cardiovascular diseases. Thus, serum cholesterol regulation has an important therapeutic role.

The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol, cholesteryl esters, or total cholesterol (which constitutes both free cholesterol and cholesteryl esters). The cholesterol concentration is determined via a coupled enzymatic reaction. This kit allows for either fluorometric or colorimetric detection, to allow maximum flexibility and a large detection range. The linear ranges are as follows:

• Fluorescence assay: 0.1–0.5 μg
• Colorimetric assay: 1–5 μg

Application

• Measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples (For example: Serum and Plasma and tissue culture).
• Measurement can be done either by fluorometric or colorimetric detection.

Features and Benefits

• Highly sensitive, simple and high-throughput assay for measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples
• User friendly: No need to weigh or calculate needed amounts of reagents on your own
• Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
• Quick instruction bench card - to assure your experimental success
• Suitable for both colorimetric and fluorometric detection
• More experiments in one kit - contains sufficient reagents for 100 colorimetric or 400 fluorometric tests.
• Detection time: only 30 minutes

Suitability

Suitable for the detection of free cholesterol, cholesteryl esters, or total cholesterol in biological samples.