General description
The most hydrophobic of the Chromolith® WP 300 line, the RP-18 column is useful for the resolution of peptides and smaller proteins. One critical quality attribute (CQA) required by regulatory bodies is the peptide map of a biotherapeutic. Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RPHPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information.
The Chromolith® WP 300 RP-18 column gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry incorporated into the Chromolith® WP 300 line improve resolution by increasing efficiency and reducing peak tailing.
Chromolith® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® WP 300 RP-18 2 mm I.D. columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Ã…), the surface modification is Octadecylsilane. Chromolith® WP 300 RP-18 columns fall under the USP L1 Classification. Chromolith® WP 300 RP-18 columns are fully suitable for LC-MS applications.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for very fast separations of Biomolecules on any UHPLC System.
Chromolith® WP 300 RP-18 50-2 HPLC columns enable:
—Completely bioinert column hardware and high biorecovery
—High-speed separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® WP 300 RP-18, 5-2 guard cartridges (3 pieces) [1.52372] with the Chromolith® Guard Cartridge Holder Bioinert 5 mm x 2 mm [1.52355]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series. [[1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41115709
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 1523710001
