General description

Biotherapeutics, for example, bio-engineered drugs and peptide therapeutics, represent the promise of new medical treatments for the future. Production costs have been falling leading to extremely high demand for suitable analytical methods for process monitoring and quality control of these biomolecules. HPLC is the preferred method of analysis, and it is therefore important to use the right column for these larger molecules.

Accurate analysis of proteins, antibodies, and large peptides requires columns with good permeability, along with better mass transfer and selectivity. In order for size-exclusion not to influence the separation, the pore size should be approximately ten times larger than the molecule being analyzed.Chromolith columns have shown great potential and superiority in comparison to standard silica. Chromolith^® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® WP 300 RP-18 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), and the surface modification is Octadecylsilane. Chromolith^® WP 300 RP-18 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith^® WP 300 RP-18 columns are suitable for LC-MS applications.The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for very fast separations of Biomolecules on any HPLC System.Chromolith^® WP 300 RP-18 50-4.6 HPLC columns enable:

Completely bioinert column hardware and high biorecovery
High-speed separations at very low column back-pressure
High matrix tolerance, allowing time and cost savings with simplified workflows
Cost-savings due to extended column lifetimes
Connection of columns, flow gradients, and the use of mobile phases with higher viscosity

The recommended Guard-columns are: Chromolith^® WP 300 RP-18, 5-4.6 guard cartridges (3 pieces) [1.52273] with the Chromolith^® Guard Cartridge Holder Bioinert 5 mm x 4.6 mm [1.52255] Or Chromolith^® WP 300 RP-18 10-4.6 guard cartridges (3 pieces) [1.52272] with the Chromolith^® Guard Cartridge Holder Bioinert 10 mm x 4.6 mm [1.52256]. The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series. [1.51467] Learn more: Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC ColumnsChromolith^® WP 300 RP-18 columns are also available with 2 mm inner dimension. Flyer: Rapid and Robust Protein Chromatography - Chromolith^® WP 300 RP-18 2 mm I.D. HPLC Columns. All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments. Supelco HPLC Products: The Best Choice for Any LC Instrument