General description
Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.
Application
Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.
Quality
Contaminant: <0.5% free 4-nitraniline
Sequence
Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
Principle
Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.
Preparation Note
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Chromozym is a registered trademark of Pentapharm AG, Basel
- UPC:
- 41141612
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10378445001
