Citrate Assay Kit (C007B-105002)

DescriptionCitrate is a key Tricarboxylic Acid (TCA) cycle intermediate formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA. Citrate is transported out of the mitochondria via the citrate-malate shuttle and converted back to acetyl-CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis via its actions on acetyl-CoA carboxylase and of glycolysis via its actions on phospho- fructokinase. Citrate metabolism and disposition can vary widely due to sex, age, and a variety of other factors including disease states. Cellular citrate levels are decreased in prostrate cancer cells and citrate levels may be a marker of prostrate cancer growth rate. The Citrate Assay Kit provides a simple, sensitive, and rapid means of quantifying citrate in a variety of samples.Citrate Assay kit has been used to determine the concentration of citrate in samples.Suitability: Suitable for the measurement of citrate in a variety of samples including tissue and cellsPrinciple: Citrate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex= 535/λem= 587 nm) product, proportional to the citrate present. Typical sensitivites of the Citrate Assay Kit are between 0.1 to 10 nmoles (2 μM-10 mM) of citrate in a variety of samples. Specification: sufficient for 100colorimetricorfluorometrictests Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C486306-SCI_d6084b6de2924eb47f20532ff5af72be.pdf