Application
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs
Biochem/physiol Actions
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Unit Definition
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
Preparation Note
Dissolves in water to form a clear solution at 1 mg/mL concentration.
- UPC:
- 51123707
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- C3260-1KU
