CLASSIC IMMUNOPRECIPITATION 50

Immunoprecipitation (IP) is one of the most useful immunochemical techniques.IPs are routinely used to determinethe presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interactions with other proteins, nucleic acids and ligands. IPs consist of four main steps: Labeling of the antigen (Optional step) Release of antigen by cell lysis Formation of antibody-antigen complexes Purification of the immune complexes. G-Biosciences Classic Immunoprecipitation kit contains all the reagents necessary to complete all aspects of immunoprecipitation, with the exception of labeling. The kit is supplied with Immobilized Protein A/G resin , spin columns and buffers.The kit is designed for effective immunoprecipitations using <10µg antibody. The kit is suitable for 50 reactions using 10µl Immobilized Protein A/G Agarose. Features Rapid immunoprecipitations Mild elution conditions Suitable for most common immunoglobulins Applications For traditional immunoprecipitations