Cell Line Origin
Mosquito larvae, Aedes albopictus
Cell Line Description
Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.
Application
Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO3) level of 1.1g/l is recommended).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm2 . Cells are dislodged by mechanical agitation; 5% CO2; 28°C. Freeze in 10% glycerol.
Other Notes
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biological source: insect larvae. growth mode: Adherent. karyotype: Diploid. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106504
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 89051705-1VL