CMT 64

Cell Line Description

CMT 64 was isolated from a primary alveogenic lung carcinoma tumour mass in C57BL/lcrf mouse, achieving stable morphology and growth rate in culture, similar to the growth rate and morphology in the primary tumour, and in lung metastasis induced after subcutaneous inoculation of mice. CMT 64 cells can be maintained in completely defined serum-free medium. Tumours derived from tissue culture cells grown in serum-free and serum-containing medium can form lung metastases in appropriate animal host.

Application

CMT 64 has been used to study the effect of cleavage of endogenous interleukin-33 with allergen extracts and protease allergens. It has also been used in in vitro IL-33 release assay.

Culture Medium

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10⁵ cells/cm²

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: CMT 64 (ECACC 10032301)

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.