Cell Line Origin
Mouse C57BL/1CRF lung carcinoma
Cell Line Description
Derived from an alveologenic lung carcinoma from a C57BL/1CRF mouse. Can be maintained in a completely defined serum free medium, but the growth rate is much slower than in supplemented serum. Used in studies of lung metastases.
Application
Metastasis studies
Culture Medium
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
biological source: mouse lung. growth mode: Adherent. karyotype: Modal no. 85. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86082105-1VL