COLLAGEN CELL CULTURE GRADE

General description

Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens  are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.

Specificity

Active on most mammalian cells.

Application

Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.

Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.

Biochem/physiol Actions

Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.

Features and Benefits

Contents
Lyophilizate, cell culture grade, 3 vials of 10mg

Specifications

Biological activity: Tested for the promotion of adherence of HUV-EC cells.

Preparation Note

Working concentration: 5 μg/cm²
5 μg/cm² for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.

Reconstitution

IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.

Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.

Other Notes

For life science research only. Not for use in diagnostic procedures.