Anti-Collagen 1 alpha 1 telopeptide Antibody is suitable for use in ELISA, Western Blotting and IHC. The antibody works well for IHC on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix. For Western blot with appropriately prepared samples expect a band of approximately 140 kDa in size corresponding to the telopeptide portion (C-terminus) of the collagen I alpha I polypeptide. Specific conditions for reactivity should be optimized by the end user.
- UPC:
- 41106200
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 600-401-D20
