Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.Application:Collagenase from Clostridium histolyticum has been used:for the recovery of neutrophils from collagen gelsfor the dissociation of breast tumor biopsies into single cellsfor the isolation of primary human endometrial stromal cells(HESC) from endometrial biopsiesCollagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.
Specification: purified by chromatography,≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder
MDL Number: MFCD00130830
EC Number: 232-582-9
PubChem CID: 44384985
Isomeric SMILES: CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C
- UPC:
- 41105902
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- C754905-7.5KU
- CAS:
- 9001-12-1
- Product Size:
- 7.5KU
- Hazard Statement Codes:
- H334
