Collagenase NB 4G From Clostridium Histolyticum (C09-0814-961)

DescriptionCollagenase NB 4G is a crude collagenase which contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities leads to gentle and efficient tissue dissociation.Suitable for isolation of a broad variety of cell types, especially for fibroblasts, human and rodent hepatocytes, frog oocytes and epithelial cells.ApplicationCollagenase NB 4G is suitable for dissociation of a broad variety of tissue types. SpecificationsContains class I and class II collagenase (1) and a balanced mix of neutral protease, clostripain and trypsin-like activities.Activity (Wünsch): ≥ 0.18 U/mg(Clostridiopeptidase A)EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenase) • CAS [9001-12-1]References and DefinitionsUnit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. References1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-30912. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51. Specifications and Purity: Proved Grade,≥ 0.18 U/mg(PZ activity* acc. to Wünsch, 25 °C). MDL Number: MFCD00130830. EC Number: 232-582-9. PubChem CID: 44384985. Isomeric SMILES: CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C279008-SCI_38a8c9d7efb919dceff5ed4da49b553e.pdf.