Collagenase NB 7D From Clostridium Histolyticum (C007B-096575)

DescriptionCollagenase NB 7D is suitable for isolation of a broad variety of cells. Moreover, it can be used especially where sensitive conditions are favored as during isolation of dendritic and tumor cells from human or animal tissues. To meet these requirements, Collagenase NB 7D combines the benefits of slightly higher collagenase activity with low tryptic activities ideal to preserve cell-surface proteins.ApplicationCollagenase NB 7D can be used for a variety of different applications when sensitive cells are isolated.SpecificationsContains higher amounts of class I and class II collagenase (1) and lower trypsin-like activities.Activity (Wünsch): ≥ 0.2 U/mg(Clostridiopeptidase A)Trypsin-like activities BAEE): ≤ 0.2 U/mgRatio Collagenase : Trypsin: ≥ 3.0EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1] References and DefinitionsUnit definition: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.Trypsin-like activities (BAEE assay): 1 U catalyzes the cleavage of 1 µmol BAEE per minute at 25 °C, pH 8.0. Enzyme dissolved in 1 mM HCl.References1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-30912. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51. Specification: Gentle Grade,≥ 0.2 U/mg MDL Number: MFCD00130830 EC Number: 232-582-9 PubChem CID: 44384985 Isomeric SMILES: CCCCCCCC[C@H]1CC[C@@]2([C@@]1(CC[C@]3(C2CC[C@@]4([C@@]3(CC5=C(C4)N=C6C[C@]7([C@@](CCC8[C@@]7(CC[C@]9([C@]8(CC[C@@H]9CCCCCCCC)C)C)C)(CC6=N5)C)C)C)C)C)C)C Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C278996-SCI_001d30c257b18a87f73f5fa01b22018a.pdf