Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. True collagenase may cleave simultaneously across all three chains or attack a single strand. The enzyme has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. Under normal conditions the mammalian enzyme is bound to a2-macroglobulin or other serum antiproteases. Where the enzyme is functional as in the postpartum uterus, it may be obtained directly from the tissue homogenate. Mammalian collagenases split collagen in its native triple-helical conformation at a specific site yielding fragments, TCA and TCB representing 3/4 and 1/4 lengths of the tropocollagen molecule. After fragmentation the pieces tend to uncoil into random coil polypeptides. Collagenase has found widespread application in the isolation of specific cell types from attendant connective tissue. Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. The wide spectrum of activity of crude collagenase makes it especially useful as a debriding agent. Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51172415
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 0215070401
- Model Number:
- C829Q03
- Temperature Control Device:
- Yes
