COLO 205HUMAN CAUCASIAN COLON ADENOCARCINOMA (C15-1311-148)

General description

Isolated from ascitic fluid of a 70 year old Caucasian male with carcinoma of the colon. Produces carcinoembryonic antigen.

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

COLO 205, COLO 201 (Sigma product no. 87091201) and COLO 206 (Sigma product no. 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Application

tumourigenic in suitable animals

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

Other Notes

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biological source: human colon. description: Human Caucasian colon adenocarcinoma. growth mode: Suspension. karyotype: Hypertriploid modal no 71-78. morphology: Round and refractile cells in suspension. Some cuboidal cells in the monolayer. products: Carcinoembryonic antigen (CEA). receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.