Cell Line Origin
Human melanoma
Cell Line Description
Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.COLO 794 and COLO 800 (Sigma Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.
Application
Cancer studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20
Culture Medium
RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C; cells can show extensive blebs
Other Notes
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biological source: human skin. growth mode: Adherent. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51111640
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94072237-1VL