Cell Line Origin
Human malignant melanoma
Cell Line Description
Derived from the right axilla lymph node of a 43 year old male with melanoma.COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma. This has been confirmed by STR profiling; COLO 853 also has an identical STR profile to the above cell lines though this cell line is not mentioned in the literature.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human lymph. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Melanin. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41151527
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 93052606-1VL
- Temperature Control Device:
- Yes
