DescriptionBiotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.Suitability: Suitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solutionPrinciple: This Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. Specification: sufficient for 200 colorimetric assays Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C486438-SCI_356f7bf2f6471acc08a0ab98f9891d8c.pdf
- UPC:
- 41116133
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 3.53 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- C486438-1kit
- Product Size:
- 1kit
