COLORIMETRIC MALEIMIDE ASSAY KIT (C15-1884-398)

Catalog No.: C15-1884-398
Mfr. No.: MAK162-1KT
Mfr. Name: Sigma-Aldrich
Qty/UOM: 1
UOM: KT

Price: $604.37

List Price: $671.52

General description Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 :nm is insensitive due to the

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Description
Specifications

General description

Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.

Suitability

Suitable for quantifying maleimide groups

Principle

The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.

UPC:: 51432312
Condition:: New
Availability:: 3-5 Days
Weight:: 1.00 Ounces
HazmatClass:: No
MPN:: MAK162-1KT

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