COLORWHEEL ANTIBODY-READY MFLUOR UV375 DYE

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

mFluor UV375 succinimidyl esters dye is well suited for multicolor flow cytometry applications. It exhibits a relatively larger Stokes shift and exhibit an excitation max at 351 nm and emission max at 387 nm. These spectral properties make it an ideal alternate to BUV395. However, in contrast to BUV395, which is a polymer-based dye, mFlour UV375 is a small organic molecule that case be conjugated to antibodies with ease. The NHS esters can react readily with the primary amines in proteins, amine-modified oligonucleotides, and other amine-containing molecules and the resulting dye conjugates exhibit good stability.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Application

Quality Control Testing:

Flow Cytometry: Excitation: 351 nm, Emission: 387 nm

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Antibody (Sold Separately)

Physical form

CWDS-MF375-25UL Set Components

CWD-MF375-25UL - ColorWheel^® Antibody-Ready mFluor UV375 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel^® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.