General description
Human serum that has been depleted of C1q complement component.
Human serum that has been depleted of C1q complement component. C1q-depleted human serum, sterile filtered through a 0.22 µm filter. This product has the following specifications:
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
Classical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Physical form
In PBS, ≤0.1% sodium azide, pH 7.4.
Preparation Note
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:
CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
• Recommended volume of C1q-Dpl serum per assay
• C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
• Input of purified C1q to yield 1 C1qH50:
• C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
• Input of C1q in NHS Complement standard to yield 1 C1qH50
• Ratio
• Background A412 EA blank reading at the recommended input of C1q-Dpl
CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
• CH50/ml NHS Standard
• CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
• Ratio
ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
• APH50/ml NHS Standard
• Input of NHS Standard to yield 1 APH50
• APH50/ml C1q-Dpl
• Input of C1q-Dpl to yield 1 APH50
• AP Ratio
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
form: solution. Quality Level: 100. does not contain: preservative. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. impurities: C1q, none detected (Ouchterlony using various dilutions of goat anti-human C1q antiserum.). color: clear amber. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 234401-1ML