Complement C5-Depleted Serum, Human (C15-1301-461)

General description

Human serum depleted of complement component C5 by affinity chromatography.

Human serum that has been depleted of complement component C5 by affinity chromatography. Intended for the detection and quantitation of hemolytically active C5 complement component. The classical and alternative pathways are intact except for the specifically depleted component. Recommended assay volume: ≤60 µl

Biochem/physiol Actions

Complement C5-depleted serum from humans produced by affinity chromatography aids the development of the complement cascade up to the desired convertase level. It eliminates the usual time-consuming, expensive, and stepwise formation of enzymatic complexes from purified products. Whole serum usage reduces the risk of protein inactivation during the process. It also promotes better physiological conditions. Complement C5-Depleted Serum is intended for the detection and quantitation of the hemolytically active C5 complement component. Creating complement convertases on erythrocytes enhances a better readout of the assay by spectrophotometric quantification of released hemoglobin.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 145 mM NaCl, 10 mM sodium phosphate, pH 7.3.

Preparation Note

Prepared from plasma of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

form: liquid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. impurities: C5 antigen, none detected (Ouchterlony). shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.