CompoZr(R) Targeted Integration Kit - mRosa26

General description

The CompoZr Targeted Integration Kit - mRosa26 is designed to rapidly integrate a user-specified gene of interest (GOI) into the preferred site of integration in the mouse genome, the Rosa 26 locus. The well characterized Zinc Finger Nucleases (ZFNs) in this kit target the Rosa26 locus with high specificity, creating a double strand break. ZFN treated cells then use a pZDonor plasmid with homology arms to the Rosa26 genomic locus to repair the ZFN created double stranded break by the natural repair process of homology directed repair. The pZDonor plasmid can be engineered to contain your GOI and will direct targeted integration of your GOI into the Rosa26 locus. <http://www.sigmaaldrich.com/life-science/zinc-finger-nuclease-technology/compozr-kit.html>The CompoZr Targeted Integration Kit - mRosa26 provides a common integration site within a defined locus to generate stable, uniform transgene expression levels.

Application

CompoZr^® targeted integration kit - mRosa26 has been used to target the Rosa26 locus in mouse zygotes.

Features and Benefits

Effective Integration Efficiency

• Any mouse cell type can be modified with the CompoZr Targeted Integration Kit - mRosa26
• The ZFNs are a well-validated pair that cleaves the target DNA with high specificity
• ZFN mediated targeted integration can be achieved with high success rates

No Transgene Landing Pads Required

• Prior to ZFN transfection cell lines don′t require engineering of transgene landing pads
• Lack of need for transgene landing pads enables immediate transfection of cell lines with GOI

Rapid Biallelic Integrations with a Single Transfection

• Monoallelic and biallelic integrations have been generated with ZFNs
• Gene integrations can occur in as little as 48 hours

* This kit was developed for targeted integration applications using cell lines and has not been optimized for use with embryo microinjection methods used to make genetically modified animals. For detailed information on potential applications with this kit please contact Sigma Technical Service.

Legal Information

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC