General description
Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
- as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
- for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
- for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
- for the stimulation of spleen in mitogen assays
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-
Quality
Highly purified by specific affinity chromatography
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
- UPC:
- 41202058
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- C2010-25MG