General description
Concanavalin A is a protein containing only β-sheets in its structure, and belongs to the legume lectins family.
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
- in in vitro cell proliferation assay and cell cycle assessment
- in lymphocyte proliferation
- in splenocyte culturing
Biochem/physiol Actions
Concanavalin A is known to stimulate acute hepatic inflammation and acts as a Tâ€cell mitogen. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy.
Con A is not blood group specific but has an affinity for terminal α-
Analysis Note
A lymphocyte transformation assay is used to determine suitability for cell culture applications. The mitogenic activity of this product is assessed by bromodeoxyuridine incorporation and is found to have peak incorporation at ≤75 ug lectin/ml of medium.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
- UPC:
- 41202058
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- C5275-5MG