General description

Cortisol or hydrocortisone, also known as the stress hormone, is aglucocorticoid steroid. It is released by the adrenal glands and is involved inmaintaining the circadian rhythm. The hormone is measured in whole blood by LC-MS/MS for newborn screening of congenital adrenal hyperplasia (CAH) and in serum or plasma by LC-MS/MS for determination of several diseases including Cushing’s syndrome, Addison’s disease, and Sheehan’s syndrome.

Application

This Certified Reference Material (CRM) in solution can also be used as follows:

Development of a disposable immunosensor based on direct competitive-enzyme linked immunoassay (DC-ELISA) to determine cortisol in human saliva samples
Quantitative analysis of cortisol in human hair samples by a liquid-chromatography-tandem mass spectrometric (LC-MS/MS) method
Selective and sensitive analysis of both cortisol and cortisone in human hair samples by an automated solid phase extraction (SPE) method combined with liquid chromatography (LC) coupled to electrospray ionization-tandem mass spectrometry (ESI-MS³)
Multi-residue determination of cortisol, cortisone, and their three tetrahydro metabolites from human urine samples by an LC-MS/MS method
Human saliva analysis for the estimation of cortisol and cortisone by ionic liquid dispersive liquid-liquid microextraction (IL-DLLME) and liquid chromatography combined with ultra-violet/visible (UV/Vis) detection

Features and Benefits

Fully characterized under ISO/IEC 17025 and ISO 17034 accreditation
Accompanied with a comprehensive Certificate of Analysis (CoA) with data on stability, homogeneity, accuracy of concentration, uncertainty, and traceability
Rigorously tested through real-time stability studies to ensure accuracy and shelf life
Gravimetrically prepared using qualified precision balances to ensure minimal uncertainty
Flame sealed under argon into ampoules for long-term shelf life
Offered in a convenient, DEA-exempt format to improve laboratory efficiency