CPGENOME 5-HMC DNA STANDARD

General description

CpGenome 5-hmC DNA Standard is a linear, double-stranded DNA (897 bp) containing 100% 5-hydroxymethylcytosine. This standard can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. This vial is a component of S8005 the CpGenome 5-mC and 5-hmC DNA Standard Set ( three linear, double-stranded DNAs (897 bp) with the same sequence, but containing either 100% 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines.

Materials Provided:

One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.

Validation:

Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.

Application

Research Category
Epigenetics & Nuclear Function

Physical form

Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Storage and Stability

Recommended Storage: Stable for 6 months at -20°C from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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