Cell Line Origin
Cat kidney
Cell Line Description
CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.
Application
Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: feline kidney. growth mode: Adherent. karyotype: Hypodiploid, modal no 36. morphology: Epithelial. products: Carcinoembryonic antigen (CEA). receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41141948
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 86093002-1VL
