Cell Line Origin
Rat NEDH islet tumour
Cell Line Description
Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (Sigma Catalogue number. 88031604).
Application
Hormone studies
Culture Medium
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The cells tend to grow in clumps and ribbons.
Other Notes
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biological source: rat pancreas. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Insulin and glucagon. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 88031603-1VL