General description
A cell-permeable butenediamide compound that acts as a potent, active site cysteine reactive irreversible inhibitor of CRM1/exportin 1. Shown to selectively repress agonist-mediated nuclear export of HDAC4/5, Rev and NFAT proteins in neonatal rat ventricular myocytes (NRVMs; EC50 = 2.2 and 3.5 nM for GFP-HDAC5 and GFP-HIV Rev) and suppress hypertrophic growth of NRVMs (EC50 = 52 nM for ANF expression inhibition) with negligible effect either on the phosphorylation of HDAC5 or on the kinase activities of PKD and CaMKII at 1 µM.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Monovich, L., et al. 2009. Biochim Biophys Acta.1789, 422.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 379.64. Empirical Formula: C16H12BrClN2O2. Quality Level: 100. Assay: ≥. 95% (HPLC). form: powder. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, protect from light. color: yellow. solubility: DMSO: 100 . mg/mL. shipped in: wet ice. storage temp.: 2-8°C. SMILES string: O: C(NC1: CC: CC(Cl): C1)/C: C\C(NC2: CC: C(Br)C: C2): O. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51124303
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 238100-10MG