CSPD 1ML

General description

CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

Application

CSPD ready-to-use has been used for:

• the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
• colony and plaque hybridization
• gel shift assay
• sequencing

It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.

Features and Benefits

• Save time with a fast, highly sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.
• Reuse the substrate by filtering and storing in sodium azide.

Physical form

25mM solution (11.6mg/ml), 100x concentrated, colorless solution.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.