CVF-NHS

General DescriptionCVF-NHS is generated by activating the complement system in normal human serum with cobra venom factor (CVF) purified from the venom of Naja naja kaouthia. CVF from Naja naja kaouthia produces an enzyme that cleaves both human C3 and C5. The affinity of the Naja naja kaouthia CVF for C5 is so high that it primarily consumes C5 first, then C3 ((Rawal, N. and Pangburn, M.K. (2000)).Physical Characteristics & StructureCVF-NHS is serum supplied as a clear, straw-colored liquid containing an activated complement system and hence no complement activity. CVF-NHS also contains CVF used for activating the complement system.FunctionCVF-NHS is tested for C3 and C5 activity by hemolytic assays using antibodysensitized sheep erythrocytes and C3-depleted human serum or C5-depleted human serum. The assays verify that CVF-NHS has < 10% of C3 and C5 activity or >90% consumption of C3 and C5 activity in CVF-NHS when compared to normal human serum standard.AssaysSee FunctionApplicationsCVF-NHS is a serum source which lacks complement activity due to its consumption upon activation with CVF.Precautions/Toxicity/HazardsThe source is human serum, therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II.Hazard Code: B WGK Germany 3MSDS available upon request. Specifications and Purity: >50 mg/ml,0.22 µm filtered. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/C501297-SCI_42daeb4ce939302e9ada6387d1432a8f.pdf.