CWP232228 (C09-1120-256)

CWP232228, a highly potent selective Wnt/β-catenin signaling inhibitor, antagonizes binding of β-catenin to T-cell factor (TCF) in the nucleus. CWP232228 suppresses tumor formation and metastasis without toxicity through the inhibition of the growth of breast and liver cancer stem cells (CSCs)In VitroCWP232228 (0.01-100 μM; 48 hours) inhibits cell proliferation with IC 50 values are 2 and 0.8 μM in mouse (4T1) and human (MDA-MB-435) breast cancer cell lines, respectively. CWP232228 (0.01-10 μM; 48 hours) inhibits cell proliferation with IC 50 s of 2.566, 2.630, and 2.596 μM in Hep3B, Huh7 and HepG2 cells, respectively. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: Mouse (4T1) and human (MDA-MB-435) breast cancer cell lines Concentration: 0.01, 0.1, 1, 10, 100 μM Incubation Time: 48 hours Result: IC 50 s were 2 and 0.8 μM for 4T1 and MDA-MB-435 cell lines, respectively. Cell Proliferation AssayCell Line: Hepatocellular carcinoma cell lines HepG2, Huh7, and Hep3B Concentration: 0.01, 0.1, 0.5, 1, 5, 10 μM Incubation Time: 48 hours Result: IC 50 s were 2.566, 2.630, and 2.596 μM for Hep3B, Huh7 and HepG2 cells, respectively.In VivoCWP232228 (100 mg/kg, administered i.p.; daily; 21days for mice bearing 4T1 cell tumors; 60 days for mice bearing MDA-MB-435 cell tumors) results in a significant reduction in tumor volume . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 7-week-old female Balb/c and NOD/SCID mice bearing 4T1 or MDA-MB-435 cell tumors Dosage: 100 mg/kg Administration: Administered i.p.; daily; 21days for mice bearing 4T1 cell tumors, 60 days for mice bearing MDA-MB-435 cell tumors Result: Treatment resulted in a significant reduction in tumor volume.Form:SolidIC50& Target:Wnt/β-catenin. Specifications and Purity: 98%. Molecular Formula: C33H34N7Na2O7P. Molecular Weight: 717.62. PubChem CID: 71509158. Isomeric SMILES: CN1C=C2C=CC=C(C2=N1)CN3C[C@H]4N([C@H](C3=O)CC5=CC=C(C=C5)OP(=O)([O-])[O-])C(=O)CN(N4C(=O)NCC6=CC=CC=C6)CC=C.[Na+].[Na+].