F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of goat IgG heavy chain but not with the Fab portion of goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species. Target: Goat Host: Rabbit Antibody Format: F(ab')2 Fragment Specificity: IgG, Fc Fragment Specific Minimal Cross Reactivity: Human Serum Proteins Conjugate: Cyanine Cy™3 Clonality: Polyclonal BSA and dry milk may contain IgG which will be recognised by this antibody. Use of BSA or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titre. This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Cy is a trademark of Cytiva Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by Cytiva Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.
- UPC:
- 41106200
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazMatClass:
- Yes
- WeightUOM:
- LB
- MPN:
- 305-166-046
