Application
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.
Biochem/physiol Actions
Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Features and Benefits
- Simple, optimized protocol - Obtain reproducible results without special training needs
- Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
- Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
- May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
- Use to analyze the intactness of mitochondrial membranes
- Simple colorimetric measurement of solution
- UPC:
- 41116111
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CYTOCOX1-1KT
