Anti-Dab1 antibody is suitable for the detection of Dab1 by immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate. For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature. For immunoprecipitation use 1 µl of antiserum per 500 µg of brain lysate. Perform immunoprecipitation at 4°C for 2 h. For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 µg/ml aprotinin and 10 µg/ml leupeptin.
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- UPC:
- 51202407
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 100-401-225
- Temperature Control Device:
- Yes
