General description
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cells are typically stained at 1 µg/ml.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
DNA
Reversible: yes
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Physical form
A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
Other Notes
Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.
Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.
Russels, W.C., et al. 1975. Nature253, 461.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51131613
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 5087410001
