DAPI staining solution (ready to use)

Product introduction：DAPI is a nuclear staining reagent that can stain DNA. It releases blfluorescence after embedding double stranded DNA, and the brightness is enhanced about 20 times. DAPI is often used for apoptosis detection, and it is observed by fluorescence microscope or detected by flow cytometry after staining. DAPI has a high photobleaching tolerance and can be used to detect yeast mitochondrial DNA, chloroplast DNA, viral DNA and stained DNA. Take adherent cells (96 well plate) as an example, 100 per well μ L staining working solution, 10 ml can be used for the staining of 100 wells.Product parameters：Ex/em (bound DNA) =360/460 nmcas No.: 28718-90-3 molecular weight: 350.3 molecular formula: c16h17cl2n5Matters needing attention： 1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. the concentration of this dyeing solution is optimized to meet the needs of various conventional dyeing. If you need to adjust the concentration, please select D489987 or D106471 ，and configure the appropriate working fluid concentration by yourself. 3. DAPI is harmful to human body. Please wear experimental clothes and disposable gloves for operation, and pay attention to proper protection. 4. fluorescent dyes have quenching problems. Please try to avoid light to slow down quenching. Scope of application：Nuclear staining Instruction：For cell or tissue samples, after fixation, appropriate washing to remove the fixative. If immunofluorescence staining is performed, DAPI staining is performed after staining. If no other staining is required, subsequent DAPI staining is performed directly. 1.For adherent cells ( remove the medium in the orifice plate ) or tissue sections, add a small amount of DAPI staining solution and cover the sample. For suspension cells, at least 3 times the volume of the sample to be stained was added and mixed. 2.Cells were cultured at room temperature for 10-20 min. 3.The cells were washed twice with PBS or appropriate buffer, 3-5 min each time, and 50 μL PBS was added after washing to prevent the cells from drying out. 4.Cells were observed under a fluorescence microscope with a filter with 360 nm excitation wavelength and 460 nm emission wavelength. Molecular Formula: C16H17Cl2N5 Molecular Weight: 350.25 EC Number: 249-186-7 PubChem CID: 160166 Isomeric SMILES: C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl Beilstein Registry Number: 4894417