ddNTP Mix (C007B-555487)

ddNTP Mix (10mM each, Suitable for MALDI-MS) is a mixture of ddATP, ddCTP, ddGTP and ddTTP at 10mM each, specifically designed for MALDI-TOF MS analysis. ddNTPs are Mass-Modified Terminators and can be used for mass spectrometry analysis after single base primer extension. The ddTTP is specially modified to have a larger molecular weight than regular ddTTP. ddATP, ddCTP, ddGTP and ddTTP after incorporation into DNA have distinguishable molecular weights of approximately 271, 247, 287, and 327 Da, respectively, facilitating high-throughput single nucleotide polymorphism (SNP) analysis by MassArray mass spectrometry.With the discovery of several matrices capable of ionizing DNA and RNA, MALDI-TOF MS has become a powerful toll for the study of nucleic acids. It can be used to detect sequence variations such as mutations, single nucleotide polymorphisms (SNPs), insertions/deletions, and alternative splicing, as well as quantitative changes of gene copy numbers, gene expression, and allelic expression. Additionally, it can also be used to detect the methylation of genomic DNA and post-transcriptional modifications of tRNA and rRNA [1].ddNTP (Dideoxynucleoside Triphosphate) contains a dideoxy ribose group and lacks a 3' OH group. Therefore, unlike dNTP, it cannot form a phosphodiester bond during DNA synthesis or amplification, leading to chain termination.The molecular weight difference between the products generated by ordinary ddNTP after single base primer extension ranges from 9-40 Da, making it difficult to perform high-throughput genotyping by mass spectrometry. The ddTTP in this product has been specially modified to have a larger molecular weight than regular ddTTP, resulting in a molecular weight difference of 15 Da or more in the products, which allows genotyping based on the mass difference of the terminal target base [2-3]. The approximate mass differences (Da) of the bases are shown in the table below. Terminator A C G T A 0 -24 16 56 C 24 0 40 80 G -16 -40 0 40 T -56 -80 -40 0 This product is designed specifically for MALDI-MS or MALDI-TOF-MS analysis. It is a mass-grade product with high purity (HPLC ≥99%), containing ddATP, ddCTP, ddGTP, and ddTTP at 10mM each. This product has a pH of 7.4±0.1 and does not contain any nucleases.Precautions：This product can be thawed at room temperature. After thawing, keep it on ice for use and store at -20℃ immediately after use.Nuclease-free pipette tips and centrifuge tubes should be used for the experiments.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：Please refer to the instructions of MassArray or other assays for the usage of this product.References:1.Edwards JR, Ruparel H, Ju J. Mutat Res. 2005. 573(1-2):3-12. 2.Oeth P, Beaulieu M, Park C, Kosman D, et al. Sequenom Appl Note. 2005. 27:8876-006.3.Millis MP. Methods Mol Biol. 2011. 700:61-76. Related Products：Cat. No.Product NamePack SizeD7366dNTP (4 tubes, 100mM)4×50μlD7371dNTP Mixture (2.5mM each)1mlD7373dNTP Mixture (25mM each)250μlD7401-100μlddNTP Mix (10mM each, Suitable for MALDI-MS)100μlD7401-500μlddNTP Mix (10mM each, Suitable for MALDI-MS)500μlD7401-2mlddNTP Mix (10mM each, Suitable for MALDI-MS)2mlST876-100ml Ultrapure Water (DNase/RNase-Free, Sterile)100mlST876-500ml Ultrapure Water (DNase/RNase-Free, Sterile)500ml

Specification: Suitable for MALDI-MS, 10mM each