Deacetylation Inhibition Cocktail

Deacetylation Inhibition Cocktail is a synergistic combination of chemicals designed to preserve the acetylation state of proteins including histone, tubulin, p53, and other acetyl-lysine modified targets. Lysine acetylation is an important post-translational modification that can affect protein transcription, cell cycle, and signal transduction pathways. The balance between acetyltransferase enzymes and deacetylase enzymes affects chromatin condensation, transcription factor activity and protein aggregation and is of significant interest to researchers. Deacetylation Inhibition Cocktail is a useful tool for investigating these important biological pathways.|Deacetylation Inhibition Cocktail has been developed and tested in our laboratories and has shown to effectively inhibit HDAC′s and Sirtuins in various cellular samples and applications.|Suitable for use in Western Blotting, Flow Cytometry, and ImmunoFluorescence applications.Provided in 2 ml DMSO, sufficient to treat 200 ml of culture media. Recommended induction times of 16-48 hours. Since applications vary, the optimal working dilution should be determined by the researcher.Deacetylation Inhibition Cocktailis preserves the acetylation state of acetyl-lysine modified proteins. Specification: Every 2 ml contains: 40 µM Trichostatin A (sc-3511) 1 mM EX-527 (sc-203044) 400 mM Nicotinamide (sc-208096) Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/D388027-SCI_a8172d4f636e645e7fcef2fc07299879.pdf