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DENATURATION SOLUTION FOR NORTHERN AND SOUTHERN BLOTTING (C005B-444648)

Catalog No.
C005B-444648
Mfr. No.
N1531-4L
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
EA
Price: $342.68
List Price: $380.76

General description Sigma′:s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination

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General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Components

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

Other Notes

0.5 N NaOH, 1.5 M NaCl

Shipping Information:

Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.

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UPC:
41105514
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
Yes
MPN:
N1531-4L

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