Deoxycholate Citrate Agar with 1.5% Agar, 500G

Deoxycholate citrate agar w/1.5% agar is a selective medium recommended for the isolation of enteric pathogens. Sodium deoxycholate, sodium citrate and ferric citrate are inhibitory agents in the medium. Lactose is the fermentable carbohydrate. Lactose fermenters utilize lactose and produce acidic conditions around the lactose fermenting colony. This acidity causes the pH indicator, neutral red, to change its color to red. The reduction of sodium thiosulphate to sulfide is indicated by the formation of black iron sulfide. Salmonella and Shigella species do not ferment lactose but Salmonella may produce H2S forming colorless colonies with or without black centers. Escherichia coli produces pink colored colony w/bile precipitate, Enterobacter aerogenes produce pink colony. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis for Invitro Diagnostic Use Appearance: Light yellow to pink homogeneous free flowing powder Gelling: Firm, comparable with 1.5% Agar gel Color and Clarity of prepared medium: Reddish orange colored, clear to slightly opalescent gel forms in Petri plates Reaction: Reaction of 4.85% w/v aqueous solution at 25 deg C. pH : 7.0±0.2