Deoxyribonuclease I from Bovine Pancreas(RNase & Protease Free,Solution) (C09-0835-679)

BbpDNase I is not base nor sequence specific; however, it does not cleave randomly. It shows preference for cleavage at the 5’ side of pyrimidines, and is particularly pronounced in alternative copolymers (Bernardi et al. 1975, and Lomonossoff et al. 1981). It has been shown that variations in the twist angle are recognized by DNase I (Dickerson and Drew 1981). The specificity of DNase I also depends on the divalent cations present. In the presence of Ca2+ and Mg2+, it causes single strand breaks, and in the presence of Mn2+ double strand breaks have been reported (Junowicz and Spencer 1973, and Campbell and Jackson 1980).Protein Accession Number: P00639CATH Classification (v. 3.2.0):Class: Alpha BetaArchitecture: 4-Layer SandwichTopology: Deoxyribonuclease I; Chain A Molecular Weight: 29.1 kDa (Theoretical)Optimal pH: 7.8Isoelectric Point: 5.08 (Theoretical)Extinction Coefficient: 36,750 cm-1 M-1E1%,280 = 11.1Active Site Residues:Tyrosine (Y87)Glutamate (E100)Histidine (H156)Activators:Bivalent metal ions (Junowicz and Spencer 1973b, Poulos and Price 1972, Price 1969, and Price 1975)Inhibitors:2-mercaptoethanol (Laskowski 1971) 2-nitro-5-thiocyanobenzoic acid (Moore 1981)Actin (Mannherz et al. 2008, and Lazarides and Lindberg 1974)Alfatoxin B2a, G2, G2a, and M1 (non-competitive) (Schabort 1970)EGTA (Moore 1981) and EDTA (Junowicz and Spencer 1973)Sodium dodecyl sulfate (Liao 1975a)Calf spleen inhibitor protein (Laskowski 1971)Carbodiimide and cholesterol sulfate (Iwamori 2000)Iodoacetate (Moore 1981) Applications:DNA removal in primary cell isolation: decreases viscosity providing better yieldsDNA removal in bioprocessing applicationsRemoving genomic DNA from RNA preparations prior to RT-PCRin vitro transcriptionNick translationDNase footprintingActin bindingUV crosslinking of proteins to nucleic acidsRadioactive labeling. Specifications and Purity: ≥2,000 Kunitz units/ml. MDL Number: MFCD00130918. EC Number: 232-667-0. PubChem CID: 597667. Isomeric SMILES: CN(C)C1=NC(=NC(=N1)N)C(CC2=CNC3=CC=CC=C32)NC(=O)C4=CC=CC=C4.