Deoxyribonucleic acid from calf thymus (C15-1292-379)

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Specificity

42% GC; T_m = 87 Â°C.
% GC is the percentage of G/C base pairs. T_m is the temperature at the midpoint of the thermal denaturation profile.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Features and Benefits

• No nuclease activity detected after 16 h at 37 Â°C incubation
• High quality template DNA

Preparation Note

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A₂₆₀ units per mL. ~20 A₂₆₀ units per mg DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.