Deoxyribonucleic acid, single stranded from salmon testes (C15-1258-109)

General description

Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.

Application

Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:

• in situ hybridization histochemistry of rat brain, human kidney sections and renal biopsy tissues
• preparing standard curve of DNA
• prehybridization solution in southern and northern hybridizations
• dilution of the cDNA
• fluorescent in-situ hybridization (FISH)
• ChIP analysis
• a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography
• a component of hybridization solution in FISH
• a control in DNA-DNA hybridization experiment between various bacterial species
• to precipitate probes in fluorescence in situ hybridization (FISH)

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Suitable for use as a blocking agent in Southern hybridizations.

Preparation Note

This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Other Notes

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.