Cell Line Origin
Human Caucasian skin Down syndrome
Cell Line Description
Derived from a female caucasian infant with Down Syndrome. Karyotypic analysis shows 90% of the cell population possesses 47 chromosomes with 5 chromosomes belonging to G group. These findings persist essentially unchanged throughout at least 30 serial subcultures.
Application
Chromosome studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,13
D16S539: 13
D5S818: 11,13
D7S820: 9,11
THO1: 8,9
TPOX: 8,10
vWA: 16,18
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. A further 15-20 passages expected.
Other Notes
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biological source: human skin. growth mode: Adherent. karyotype: Aneuploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41105328
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 91031102-1VL
